ALKBH5 Recombinant Rabbit Monoclonal Antibody [PSH01-16]
cat.: HA721628
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH01-16 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ALKBH5 aa 51-350 / 394. |
| Positive control: | HEK-293 cell lysate, Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, F9 cell lysate, PC-12 cell lysate, rat testis tissue lysate, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Nucleus speckle. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q6P6C2 Human | Q3TSG4 Mouse | D3ZKD3 Rat |
| Alternative names: | ABH5 AlkB, alkylation repair homolog 5 (E. coli) AlkB, alkylation repair homolog 5 Alkylated DNA repair protein alkB homolog 5 Alpha ketoglutarate dependent dioxygenase alkB homolog 5 OFOXD OFOXD1 Oxoglutarate and iron-dependent oxygenase domain containing Probable alpha ketoglutarate dependent dioxygenase ABH5 RNA demethylase ALKBH5 |
Images
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Fig1:
Western blot analysis of ALKBH5 on different lysates with Rabbit anti-ALKBH5 antibody (HA721628) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: MCF7 cell lysate (20 µg/Lane) Lane 6: F9 cell lysate (20 µg/Lane) Lane 7: PC-12 cell lysate (20 µg/Lane) Lane 8: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 44 kDa Observed band size: 35~44 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721628) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-ALKBH5 antibody (HA721628) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721628) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-ALKBH5 antibody (HA721628) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721628) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.