GRIK2 Recombinant Rabbit Monoclonal Antibody [JE39-33]
cat.: HA721630
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE39-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 103 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GRIK2 aa 116-165 / 908. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cell membrane, Postsynaptic cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:1,000 |
| Uniprot #: | SwissProt: Q13002 Human | P39087 Mouse | P42260 Rat |
| Alternative names: | bA487F5.1 EAA4 Excitatory amino acid receptor 4 GLR 6 GLR6 GluK2 GLUK6 GLUR 6 GluR-6 GluR6 Glutamate receptor 6 Glutamate receptor glutamate receptor form A glutamate receptor form B glutamate receptor form C glutamate receptor form D glutamate receptor form E Glutamate receptor ionotropic kainate 2 GRIK 2 GRIK2 GRIK2 protein GRIK2_HUMAN ionotropic kainate 2 MRT6 |
Images
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Fig1:
Western blot analysis of GRIK2 on different lysates with Rabbit anti-GRIK2 antibody (HA721630) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (10 µg/Lane) Lane 2: Mouse brain tissue lysate (70℃ heat) (10 µg/Lane) Lane 3: Rat brain tissue lysate (10 µg/Lane) Predicted band size: 103 kDa Observed band size: 103 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721630) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GRIK2 antibody (HA721630) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721630) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GRIK2 antibody (HA721630) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721630) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GRIK2 antibody (HA721630) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721630) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.