Phospho-mTOR (S2481) Recombinant Rabbit Monoclonal Antibody [JE59-62]
cat.: HA721632
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE59-62
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 289 kDa
Isotype: IgG
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser2481 of human mTOR.
Positive control: HeLa whole cell lysate, HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate.
Subcellular location: Endoplasmic reticulum membrane, Golgi apparatus membrane, Mitochondrion outer membrane, Lysosome, Cytoplasm, Nucleus, Microsome membrane, Lysosome membrane, Cytoplasmic vesicle, phagosome.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: P42345 Human
Alternative names: dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) FK506 binding protein 12 rapamycin associated protein 1 FK506 binding protein 12 rapamycin associated protein 2 FK506 binding protein 12 rapamycin complex associated protein 1 FK506-binding protein 12-rapamycin complex-associated protein 1 FKBP rapamycin associated protein FKBP12 rapamycin complex associated protein FKBP12-rapamycin complex-associated protein 1 FKBP12-rapamycin complex-associated protein FLJ44809 FRAP FRAP1 FRAP2 Mammalian target of rapamycin Mechanistic target of rapamycin mTOR MTOR_HUMAN OTTHUMP00000001983 RAFT1 Rapamycin and FKBP12 target 1 Rapamycin associated protein FRAP2 Rapamycin target protein 1 Rapamycin target protein RAPT1 Serine/threonine-protein kinase mTOR
Images
HA721632_1.jpg Fig1: Western blot analysis of Phospho-mTOR (S2481) on different lysates with Rabbit anti-Phospho-mTOR (S2481) antibody (HA721632) at 1/5,000 dilution.

Lane 1: HeLa whole cell lysate
Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 289 kDa
Observed band size: 289 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721632) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.