Phospho-Chk2 (T68) Recombinant Rabbit Monoclonal Antibody [JE43-12]
cat.: HA721633
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE43-12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Thr68 of human Chk2. |
| Positive control: | HeLa treated with 20μM Etoposide for 2 hours cell lysate, Jurkat treated with 25μM Etoposide for 5 hours cell lysate, NIH/3T3 treated with 25μM Etoposide for 5 hours cell lysate, C6 treated with 25μM Etoposide for 5 hours cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: O96017 Human | Q9Z265 Mouse Entrez Gene: 114212 Rat |
| Alternative names: | CDS 1 Cds1 Cds1 homolog Checkpoint kinase 2 Checkpoint like protein CHK2 CHEK 2 Chek2 Chk 2 CHK2 checkpoint homolog (S. pombe) CHK2 checkpoint homolog CHK2_HUMAN hCds1 HuCds 1 LFS 2 LFS2 PP1425 RAD 53 RAD53 Rad53 homolog Serine/threonine protein kinase Chk2 Serine/threonine-protein kinase Chk2 |
Images
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Fig1:
Western blot analysis of Phospho-Chk2 (T68) on different lysates with Rabbit anti-Phospho-Chk2 (T68) antibody (HA721633) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 20μM Etoposide for 2 hours cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 25μM Etoposide for 5 hours cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 25μM Etoposide for 5 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721633) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-Chk2 (T68) on different lysates with Rabbit anti-Phospho-Chk2 (T68) antibody (HA721633) at 1/1,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 25μM Etoposide for 5 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721633) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.