Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | PSH01-20 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 42 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human POLDIP2 aa 31-368 / 368. |
Positive control: | HepG2 cell lysate, HL-60 cell lysate, U-87 MG cell lysate, rat spleen tissue lysate, rat thymus tissue lysate, rat kidney tissue lysate, rat lung tissue lysate. |
Subcellular location: | Mitochondrion matrix, Nucleus. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q9Y2S7 Human Entrez Gene: 287544 Rat |
Alternative names: | 38 kDa DNA polymerase delta interaction protein delta interacting protein 2 DNA polymerase delta interaction protein p38 p38 PDIP 2 PDIP 38 PDIP2 PDIP2_HUMAN PDIP38 POLD 4 POLD4 Poldip2 Polymerase (DNA directed) delta interacting protein 2 Polymerase delta interacting protein 38 Polymerase delta-interacting protein 2 |
Fig1:
Western blot analysis of POLDIP2 on different lysates with Rabbit anti-POLDIP2 antibody (HA721637) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HL-60 cell lysate (20 µg/Lane) Lane 3: U-87 MG cell lysate (20 µg/Lane) Lane 4: Rat spleen tissue lysate (40 µg/Lane) Lane 5: Rat thymus tissue lysate (40 µg/Lane) Lane 6: Rat kidney tissue lysate (40 µg/Lane) Lane 7: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 8 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721637) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |