PPP2R5D Recombinant Rabbit Monoclonal Antibody [PSH01-21]
cat.: HA721638
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, FC
Clonality: Monoclonal
Clone number: PSH01-21
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 70 kDa
Isotype: IgG
Immunogen: Recombinant protein within human PPP2R5D aa 107-602 / 602.
Positive control: A431 cell lysate, HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, 293T cell lysate, Jurkat cell lysate, human brain tissue lysate, A431.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  FC
1:1,000
1:100
1:500-1:1,000
Uniprot #: SwissProt: Q14738 Human
Alternative names: 2A5D_HUMAN B'delta B56D Delta isoform of regulatory subunit B56, protein phosphatase 2A MGC2134 MGC8949 OTTHUMP00000039821 PP2A B subunit B' delta isoform PP2A B subunit B56 delta isoform PP2A B subunit isoform B''-delta PP2A B subunit isoform B56-delta PP2A B subunit isoform PR61-delta PP2A B subunit isoform R5-delta PP2A B subunit PR61 delta isoform PP2A B subunit R5 delta isoform PPP2R5D Protein phosphatase 2 regulatory subunit B (B56) delta isoform Protein phosphatase 2 regulatory subunit B delta isoform Protein phosphatase 2 regulatory subunit B' delta Serine threonine protein phosphatase 2A 56 kDa regulatory subunit delta isoform Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform TEG-271 Tex271
Images
HA721638_1.jpg Fig1: Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (HA721638) at 1/1,000 dilution.

Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: MCF7 cell lysate (20 µg/Lane)
Lane 5: 293T cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: Human brain tissue lysate (40 µg/Lane)

Predicted band size: 70 kDa
Observed band size: 56~70 kDa

Exposure time: 2 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721638) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721638_2.jpg Fig2: Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (HA721638) at 1/1,000 dilution.

Lane 1: Human PPP2R5C recombinant protein aa (aa1-524) 50ng
Lane 2: Human PPP2R5D recombinant protein aa (aa107-602) 50ng

Exposure time: 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721638) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721638_3.jpg Fig3: Immunocytochemistry analysis of A431 cells labeling PPP2R5D with Rabbit anti-PPP2R5D antibody (HA721638) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PPP2R5D antibody (HA721638) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721638_4.jpg Fig4: Flow cytometric analysis of A431 cells labeling PPP2R5D.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721638, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.