Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | PSH01-24 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 24 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within mouse FGF18 aa 1-207 / 207. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: O76093 Human | O89101 Mouse |
Alternative names: | FGF 18 FGF-18 Fgf18 FGF18_HUMAN FGFI Fibroblast growth factor 18 zFGF5 |
Fig1:
Western blot analysis of FGF18 on different lysates with Rabbit anti-FGF18 antibody (HA721641) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T overexpress mouse FGF18 whole cell lysate Lane 3: 293T overexpress human FGF18 whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721641) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |