LI Cadherin Recombinant Rabbit Monoclonal Antibody [PD00-49]
cat.: HA721643
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IF-Tissue
Clonality: Monoclonal
Clone number: PD00-49
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 92 kDa
Isotype: IgG
Immunogen: Synthetic peptide.
Positive control: Caco-2 cell lysate, LoVo cell lysate, human appendix tissue, human colon tissue, human stomach tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue
1:5,000
1:1,000
1:50-1:200
Uniprot #: SwissProt: Q12864 Human
Alternative names: BILL-cadherin CAD17_HUMAN Cadherin 16 Cadherin 16, formerly Cadherin 17 cadherin 17, LI cadherin (liver-intestine) cadherin, liver-intestine Cadherin-17 CDH16 CDH16, formerly Cdh17 FLJ26931 Formerly Cadherin 16 Formerly CDH16 HPT 1 HPT-1 cadherin human intestinal peptide-associated transporter HPT-1 human peptide transporter 1 Intestinal peptide-associated transporter HPT 1 Intestinal peptide-associated transporter HPT-1 LI-cadherin Liver Cadherin liver intestine cadherin Liver-intestine cadherin
Images
HA721643_1.jpg Fig1: Western blot analysis of LI Cadherin on different lysates with Rabbit anti-LI Cadherin antibody (HA721643) at 1/5,000 dilution.

Lane 1: Caco-2 cell lysate (20 µg/Lane)
Lane 2: LoVo cell lysate (20 µg/Lane)
Lane 3: PANC-1 cell lysate (negative) (20 µg/Lane)

Predicted band size: 92 kDa
Observed band size: 120 kDa

Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721643) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721643_2.jpg Fig2: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Appendix
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA721643, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA721643_3.jpg Fig3: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Colon
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA721643, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA721643_4.jpg Fig4: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Stomach (Intestinal metaplasia area)
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA721643, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA721643_5.jpg Fig5: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Kidney (Negative control)
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA721643, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.

Negative control: kidney (PMID: 25768256).
HA721643_6.jpg Fig6: Immunofluorescence analysis of paraffin-embedded human colon tissue labeling LI Cadherin with Rabbit anti-LI Cadherin antibody (HA721643) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721643, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HA721643_7.jpg Fig7: Immunofluorescence analysis of paraffin-embedded human stomach tissue labeling LI Cadherin with Rabbit anti-LI Cadherin antibody (HA721643) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721643, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.