HA721643

LI Cadherin Recombinant Rabbit Monoclonal Antibody [PD00-49]

cat.: HA721643

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IHC-P, IF-Tissue
Clonality:	Monoclonal
Clone number:	PD00-49

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 92 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide.
Positive control:	Caco-2 cell lysate, LoVo cell lysate, human appendix tissue, human colon tissue, human stomach tissue.
Subcellular location:	Cell membrane.
Recommended Dilutions: WB IHC-P IF-Tissue	1:1,000 1:1,000 1:50-1:200
Uniprot #:	SwissProt: Q12864 Human
Alternative names:	BILL-cadherin CAD17_HUMAN Cadherin 16 Cadherin 16, formerly Cadherin 17 cadherin 17, LI cadherin (liver-intestine) cadherin, liver-intestine Cadherin-17 CDH16 CDH16, formerly Cdh17 FLJ26931 Formerly Cadherin 16 Formerly CDH16 HPT 1 HPT-1 cadherin human intestinal peptide-associated transporter HPT-1 human peptide transporter 1 Intestinal peptide-associated transporter HPT 1 Intestinal peptide-associated transporter HPT-1 LI-cadherin Liver Cadherin liver intestine cadherin Liver-intestine cadherin

Images

Fig1: Western blot analysis of LI Cadherin on different lysates with Rabbit anti-LI Cadherin antibody (HA721643) at 1/1,000 dilution.

Lane 1: Caco-2 cell lysate
Lane 2: LoVo cell lysate
Lane 3: Human kidney tissue lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 92 kDa
Observed band size: 120 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721643) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Fig2: Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-LI Cadherin antibody (HA721643) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721643) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-LI Cadherin antibody (HA721643) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721643) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-LI Cadherin antibody (HA721643) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721643) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative tissue) with Rabbit anti-LI Cadherin antibody (HA721643) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721643) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunofluorescence analysis of paraffin-embedded human colon tissue labeling LI Cadherin with Rabbit anti-LI Cadherin antibody (HA721643) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721643, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Fig7: Immunofluorescence analysis of paraffin-embedded human stomach tissue labeling LI Cadherin with Rabbit anti-LI Cadherin antibody (HA721643) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721643, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.