Legumain Recombinant Rabbit Monoclonal Antibody [JE35-56]
cat.: HA721645
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE35-56 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Legumain aa 151-350 / 433. |
| Positive control: | HT-29 cell lysate, HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, Neuro-2a cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, mouse kidney tissue lysate, mouse placenta tissue lysate, rat kidney tissue lysate, human kidney tissue lysate, human kidney tissue, human placenta tissue, rat kidney tissue. |
| Subcellular location: | Lysosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q99538 Human | O89017 Mouse | Q9R0J8 Rat |
| Alternative names: | AEP Asparaginyl endopeptidase cysteine 1 Cysteine protease 1 EC 3.4.22.34 Legumain LGMN LGMN_HUMAN LGMN1 Protease Protease cysteine 1 (legumain) Protease cysteine 1 PRSC1 |
Images
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Fig1:
Western blot analysis of Legumain on different lysates with Rabbit anti-Legumain antibody (HA721645) at 1/1,000 dilution. Lane 1: HT-29 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: Neuro-2a cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: RAW264.7 cell lysate (20 µg/Lane) Lane 8: Mouse kidney tissue lysate (40 µg/Lane) Lane 9: Mouse placenta tissue lysate (40 µg/Lane) Lane 10: Rat kidney tissue lysate (40 µg/Lane) Lane 11: Human kidney tissue lysate (40 µg/Lane) Predicted band size: 49 kDa Observed band size: 36/46/56 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721645) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Legumain antibody (HA721645) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721645) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Legumain antibody (HA721645) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721645) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Legumain antibody (HA721645) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721645) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.