EDD Recombinant Rabbit Monoclonal Antibody [JE38-53]
cat.: HA721646
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE38-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 309 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human EDD aa 1-50 / 2,799. |
| Positive control: | Neuro-2a cell lysate, SH-SY5Y cell lysate, Jurkat cell lysate, A549 cell lysate, HEK-293 cell lysate, LNCaP cell lysate, human lung carcinoma tissue, human testis tissue, mouse testis tissue, rat testis tissue, SH-SY5Y. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:200 1:100 |
| Uniprot #: | SwissProt: O95071 Human | Q80TP3 Mouse | Q62671 Rat |
| Alternative names: | 4432411E13Rik AW549941 C77315 D030042K14 DD5 E3 identified by differential display E3 ubiquitin protein ligase, HECT domain containing, 1 E3 ubiquitin-protein ligase E3 ubiquitin-protein ligase UBR5 EDD 1 EDD EDD1 FLJ11310 HECT domain-containing 1 hHYD HYD Hyperplastic discs protein homolog Hyperplastic discs, Drosophila, homolog of KIAA0896 MGC57263 mKIAA0896 Progestin induced protein Progestin-induced protein Rat100 Ubiquitin protein ligase Ubiquitin protein ligase E3 component n recognin 5 UBR5 UBR5_HUMAN |
Images
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Fig1:
Western blot analysis of EDD on different lysates with Rabbit anti-EDD antibody (HA721646) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: Jurkat cell lysate Lane 4: A549 cell lysate Lane 5: HEK-293 cell lysate Lane 6: LNCaP cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 309 kDa Observed band size: 309 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721646) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-EDD antibody (HA721646) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721646) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-EDD antibody (HA721646) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721646) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-EDD antibody (HA721646) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721646) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-EDD antibody (HA721646) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721646) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunocytochemistry analysis of SH-SY5Y cells labeling EDD with Rabbit anti-EDD antibody (HA721646) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EDD antibody (HA721646) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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