GAS2 Recombinant Rabbit Monoclonal Antibody [JE48-38]
cat.: HA721647
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE48-38
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size:
Isotype: IgG
Immunogen: Synthetic peptide.
Positive control: Jurkat cell lysate, NIH/3T3 cell lysate, human liver tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, human stomach tissue, mouse liver tissue, rat liver tissue.
Subcellular location: Cytoplasm, cytoskeleton, stress fiber, Membrane.
Recommended Dilutions:
  WB
  IHC-P
1:1,000
1:200-1:1,000
Uniprot #: SwissProt: O43903 Human | P11862 Mouse
Entrez Gene: 499156 Rat
Alternative names: GAS 2 GAS-2 Gas2 GAS2_HUMAN Growth arrest specific 2 Growth arrest specific protein 2 Growth arrest-specific protein 2 MGC32610
Images
HA721647_1.jpg Fig1: Western blot analysis of GAS2 on different lysates with Rabbit anti-GAS2 antibody (HA721647) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: Human liver tissue lysate (40 µg/Lane)
Lane 4: Mouse liver tissue lysate (40 µg/Lane)
Lane 5: Mouse kidney tissue lysate (40 µg/Lane)
Lane 6: Rat liver tissue lysate (40 µg/Lane)

Predicted band size: 35 kDa
Observed band size: 35 kDa

Exposure time: 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721647) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
HA721647_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-GAS2 antibody (HA721647) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721647) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721647_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-GAS2 antibody (HA721647) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721647) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721647_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-GAS2 antibody (HA721647) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721647) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.