TAX1BP1 Recombinant Rabbit Monoclonal Antibody [JE34-80]
cat.: HA721648
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE34-80 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 91 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TAX1BP1 aa 740-789 / 789. |
| Positive control: | K-562 cell lysate, A549 cell lysate, 293T cell lysate, Jurkat cell lysate, mouse testis tissue lysate, rat testis tissue lysate, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Cytoplasm, Mitochondrion, Preautophagosomal structure, Cytoplasmic vesicle, autophagosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q86VP1 Human | Q3UKC1 Mouse | Q66HA4 Rat |
| Alternative names: | 1200003J11Rik 1700069J21Rik Aa1076 AA930106 CALCOCO 3 CALCOCO3 D6Ertd404e D6Ertd772e Human T cell leukemia virus type 1-binding protein Liver regeneration-related protein LRRG004 MGC94031 PRO0105 T6BP Tax1 (human T cell leukemia virus type I) binding protein Tax1 (human T cell leukemia virus type I) binding protein 1 Tax1 binding protein 1 Tax1 binding protein Tax1-binding protein 1 Tax1-binding protein 1 homolog TAX1BP1 TAX1BP1 protein tax1bp1b TAXB1_HUMAN TRAF 6 binding protein TRAF6 binding protein TRAF6 binding protein T6BP TRAF6-binding protein TRAF6-interacting protein TXBP 151 TXBP151 wu:fc20c12 wu:fc56e11 zgc:73219 zgc:77129 |
Images
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Fig1:
Western blot analysis of TAX1BP1 on different lysates with Rabbit anti-TAX1BP1 antibody (HA721648) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: Mouse testis tissue lysate (40 µg/Lane) Lane 6: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 91 kDa Observed band size: 91 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721648) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TAX1BP1 on different lysates with Rabbit anti-TAX1BP1 antibody (HA721648) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-TAX1BP1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 91 kDa Observed band size: 91 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721648) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-TAX1BP1 antibody (HA721648) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721648) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-TAX1BP1 antibody (HA721648) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721648) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.