Apo-H Recombinant Rabbit Monoclonal Antibody [JE37-02]
cat.: HA721649
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE37-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Apo-H aa 71-120 / 345. |
| Positive control: | Human liver tissue lysates. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P02749 Human |
| Alternative names: | Activated protein C binding protein Activated protein C-binding protein Anticardiolipin cofactor APC inhibitor Apo-H APOH APOH_HUMAN apolipoprotein H (beta-2-glycoprotein I) Apolipoprotein H B2G1 B2GP1 B2GPI Beta 2 glycoprotein I Beta 2 glycoprotein I precursor Beta(2)GPI Beta-2-glycoprotein 1 Beta-2-glycoprotein I BG Glycoprotein 1, beta-2 Glycoprotein I, beta-2 OTTMUSP00000003033 |
Images
|
Fig1:
Western blot analysis of Apo-H on human liver tissue lysates with Rabbit anti-Apo-H antibody (HA721649) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 38 kDa Observed band size: 38/55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721649) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Apo-H on different lysates with Rabbit anti-Apo-H antibody (HA721649) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD Apo-H cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 38 kDa Observed band size: 55 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.