LAP3 Recombinant Rabbit Monoclonal Antibody [PSH01-26]
cat.: HA721650
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH01-26 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human LAP3 aa 1-519 / 519. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, U-87 MG cell lysate, Daudi cell lysate, C6 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, human liver tissue, HEK-293 cell lysate, K-562 cell lysate, A549 cell lysate, LO2 cell lysate, human testis tissue, mouse liver tissue. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P28838 Human | Q9CPY7 Mouse | Q68FS4 Rat |
| Alternative names: | AMPL_HUMAN Cytosol aminopeptidase epididymis secretory protein Li 106 HEL-S-106 LAP 3 LAP LAP-3 Lap3 LAPEP Leucine aminopeptidase 3 Leucyl aminopeptidase PEPS Peptidase S Proline aminopeptidase Prolyl aminopeptidase |
Images
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Fig1:
Western blot analysis of LAP3 on different lysates with Rabbit anti-LAP3 antibody (HA721650) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: U-87 MG cell lysate (15 µg/Lane) Lane 4: Daudi cell lysate (15 µg/Lane) Lane 5: C6 cell lysate (15 µg/Lane) Lane 6: Mouse liver tissue lysate (30 µg/Lane) Lane 7: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: Lane 1-7 (left): 1 minute; Lane 1-7 (right): 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721650) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LAP3 antibody (HA721650) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721650) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Western blot analysis of LAP3 on different lysates with Rabbit anti-LAP3 antibody (HA721650) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: HEK-293 cell lysate (20 µg/Lane) Lane 4: U-87 MG cell lysate (20 µg/Lane) Lane 5: K-562 cell lysate (20 µg/Lane) Lane 6: A549 cell lysate (20 µg/Lane) Lane 7: LO2 cell lysate (20 µg/Lane) Lane 8: C6 cell lysate (20 µg/Lane) Lane 9: Mouse liver tissue lysate (40 µg/Lane) Lane 10: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-LAP3 antibody (HA721650) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721650) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-LAP3 antibody (HA721650) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721650) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Western blot analysis of LAP3 on different lysates with Rabbit anti-LAP3 antibody (HA721650) at 1/2,000 dilution. Lane 1: HepG2-si NT cell lysate Lane 2: HepG2-si LAP3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721650) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.