INPP4A Recombinant Rabbit Monoclonal Antibody [PSH01-27]
cat.: HA721651
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH01-27 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 0.95ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 110 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human INPP4A aa 1-150 / 977. |
| Positive control: | Jurkat cell lysate, 293T cell lysate, mouse brain tissue lysate, rat brain tissue lysate, Jurkat, rat brain tissue, mouse brain tissue. |
| Subcellular location: | Early endosome membrane, Recycling endosome membrane, Cell membrane, Nucleus, Cytoplasm, Postsynaptic density. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000-1:5,000 1:200 1:100 |
| Uniprot #: | SwissProt: Q96PE3 Human | Q9EPW0 Mouse | Q62784 Rat |
| Alternative names: | 107kDa 4-bisphosphate 4-phosphatase 9630012D15 D130048C09Rik Inositol polyphosphate 4 phosphatase type I 107kDa Inositol polyphosphate 4 phosphatase type I Inositol polyphosphate 4-phosphatase type I INP4A_HUMAN INPP4 Inpp4a OTTHUMP00000203138 OTTHUMP00000203139 OTTHUMP00000203141 OTTHUMP00000225217 R74740 TVAS1 Type I inositol 3 4 bisphosphate 4 phosphatase Type I inositol 3,4 bisphosphate 4 phosphatase Type I inositol-3 |
Images
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Fig1:
Western blot analysis of INPP4A on different lysates with Rabbit anti-INPP4A antibody (HA721651) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: 293T cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Mouse liver tissue lysate (low expression) Lane 5: Rat brain tissue lysate Lane 6: Rat liver tissue lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721651) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Jurkat cells labeling INPP4A with Rabbit anti-INPP4A antibody (HA721651) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-INPP4A antibody (HA721651) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-INPP4A antibody (HA721651) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721651) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-INPP4A antibody (HA721651) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721651) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (low expression) with Rabbit anti-INPP4A antibody (HA721651) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721651) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Western blot analysis of INPP4A on different lysates with Rabbit anti-INPP4A antibody (HA721651) at 1/2,000 dilution. Lane 1: 293T-si NT cell lysate Lane 2: 293T-si INPP4A cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721651) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.