Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat, Monkey |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | PSH01-29 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 0.91ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 83 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human HADHA aa 1-763 / 763. |
Positive control: | 293T cell lysate, HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, COS-1 cell lysate, mouse heart tissue lysate, mouse kidney tissue lysate, human kidney tissue lysate, rat liver tissue lysate. |
Subcellular location: | Mitochondrion, Mitochondrion inner membrane. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: P40939 Human | Q8BMS1 Mouse | Q64428 Rat |
Alternative names: | 3 ketoacyl Coenzyme A (CoA) thiolase alpha subunit 3 oxoacyl CoA thiolase 78 kDa gastrin binding protein 78 kDa gastrin-binding protein ECHA ECHA_HUMAN GBP HADH HADHA Hydroxyacyl Coenzyme A dehydrogenase/3 ketoacyl Coenzyme A thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit LCEH LCHAD Long chain 3-hydroxyacyl-CoA dehydrogenase Mitochondrial long chain 2 enoyl Coenzyme A (CoA) hydratase alpha subunit Mitochondrial long chain L 3 hydroxyacyl Coenzyme A dehydrogenase alpha subunit Mitochondrial trifunctional enzyme alpha subunit Mitochondrial trifunctional protein alpha subunit MTPA Thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit TP ALPHA TP-alpha Trifunctional enzyme subunit alpha mitochondrial precursor |
Fig1:
Western blot analysis of HADHA on different lysates with Rabbit anti-HADHA antibody (HA721653) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: HepG2 cell lysate Lane 4: Jurkat cell lysate Lane 5: SH-SY5Y cell lysate Lane 6: COS-1 cell lysate Lane 7: Mouse heart tissue lysate Lane 8: Mouse kidney tissue lysate Lane 9: Human kidney tissue lysate Lane 10: Rat liver tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 83 kDa Observed band size: 74 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721653) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |