HADHA Recombinant Rabbit Monoclonal Antibody [PSH01-29]
cat.: HA721653
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Monkey
Applications: WB
Clonality: Monoclonal
Clone number: PSH01-29
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 0.91ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 83 kDa
Isotype: IgG
Immunogen: Recombinant protein within human HADHA aa 1-763 / 763.
Positive control: 293T cell lysate, HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, COS-1 cell lysate, mouse heart tissue lysate, mouse kidney tissue lysate, human kidney tissue lysate, rat liver tissue lysate.
Subcellular location: Mitochondrion, Mitochondrion inner membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P40939 Human | Q8BMS1 Mouse | Q64428 Rat
Alternative names: 3 ketoacyl Coenzyme A (CoA) thiolase alpha subunit 3 oxoacyl CoA thiolase 78 kDa gastrin binding protein 78 kDa gastrin-binding protein ECHA ECHA_HUMAN GBP HADH HADHA Hydroxyacyl Coenzyme A dehydrogenase/3 ketoacyl Coenzyme A thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit LCEH LCHAD Long chain 3-hydroxyacyl-CoA dehydrogenase Mitochondrial long chain 2 enoyl Coenzyme A (CoA) hydratase alpha subunit Mitochondrial long chain L 3 hydroxyacyl Coenzyme A dehydrogenase alpha subunit Mitochondrial trifunctional enzyme alpha subunit Mitochondrial trifunctional protein alpha subunit MTPA Thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit TP ALPHA TP-alpha Trifunctional enzyme subunit alpha mitochondrial precursor
Images
HA721653_1.jpg Fig1: Western blot analysis of HADHA on different lysates with Rabbit anti-HADHA antibody (HA721653) at 1/1,000 dilution.

Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: SH-SY5Y cell lysate
Lane 6: COS-1 cell lysate
Lane 7: Mouse heart tissue lysate
Lane 8: Mouse kidney tissue lysate
Lane 9: Human kidney tissue lysate
Lane 10: Rat liver tissue lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 83 kDa
Observed band size: 74 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721653) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.