TRAF3 Recombinant Rabbit Monoclonal Antibody [PSH01-30]
cat.: HA721654
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH01-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TRAF3 301-568 / 568. |
| Positive control: | TF-1 cell lysate, THP-1 cell lysate, 293T cell lysate, Jurkat cell lysate, A549 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, rat spleen tissue lysate. |
| Subcellular location: | Cytoplasm, Endosome, Mitochondrion. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q13114 Human | Q60803 Mouse Entrez Gene: 362788 Rat |
| Alternative names: | CAP 1 CAP-1 CAP1 CD40 associated protein 1 CD40 binding protein CD40 bp CD40 receptor associated factor 1 CD40 receptor-associated factor 1 CD40-binding protein CD40BP CRAF 1 CRAF1 IIAE5 LAP 1 LAP1 LMP1 associated protein LMP1-associated protein 1 MIPT3 TNF receptor associated factor 3 TNF receptor-associated factor 3 TRAF 3 Traf3 TRAF3_HUMAN |
Images
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Fig1:
Western blot analysis of TRAF3 on different lysates with Rabbit anti-TRAF3 antibody (HA721654) at 1/1,000 dilution. Lane 1: TF-1 cell lysate (20 µg/Lane) Lane 2: THP-1 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: A549 cell lysate (20 µg/Lane) Lane 6: RAW264.7 cell lysate (20 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Lane 8: Mouse brain tissue lysate (40 µg/Lane) Lane 9: Rat spleen tissue lysate (40 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 64 kDa Observed band size: 50 kDa Exposure time: 1 minute 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721654) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.