PADI4 Recombinant Rabbit Monoclonal Antibody [PSH01-33]
cat.: HA721657
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH01-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PADI4 aa 1-663 / 663. |
| Positive control: | Human spleen tissue, mouse spleen tissue, rat lung tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Cytoplasmic granule. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q9UM07 Human | Q9Z183 Mouse | O88807 Rat |
| Alternative names: | EC 3.5.3.15 HL 60 PAD HL-60 PAD PAD 4 PAD PADI 4 PADI 5 PADI H protein Padi4 PADI4_HUMAN PADI5 PDI 4 PDI 5 PDI4 PDI5 Peptidyl arginine deiminase type IV Peptidyl arginine deiminase type V Peptidylarginine deiminase IV Protein arginine deiminase Protein arginine deiminase type 4 Protein arginine deiminase type IV Protein-arginine deiminase type IV Protein-arginine deiminase type-4 |
Images
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Fig1:
Western blot analysis of PADI4 on different lysates with Rabbit anti-PADI4 antibody (HA721657) at 1/1,000 dilution. Lane 1: 293T overexpress PADI4 whole cell lysate Lane 2: 293T whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721657) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PADI4 antibody (HA721657) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721657) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-PADI4 antibody (HA721657) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721657) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-PADI4 antibody (HA721657) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721657) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.