WIF1 Recombinant Rabbit Monoclonal Antibody [JE41-39]
cat.: HA721660
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE41-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human WIF1 aa 330-379 / 379. |
| Positive control: | HT-29 cell lysate, HeLa cell lysate, SW480 cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, mouse heart tissue lysate, mouse lung tissue lysate, rat lung tissue lysate, human lung tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9Y5W5 Human | Q9WUA1 Mouse | Q6IN38 Rat |
| Alternative names: | WIF-1 wif1 WIF1_HUMAN Wnt inhibitory factor 1 |
Images
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Fig1:
Western blot analysis of WIF1 on different lysates with Rabbit anti-WIF1 antibody (HA721660) at 1/1,000 dilution. Lane 1: HT-29 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: SW480 cell lysate (20 µg/Lane) Lane 4: Caco-2 cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: Mouse heart tissue lysate (40 µg/Lane) Lane 7: Mouse lung tissue lysate (40 µg/Lane) Lane 8: Rat lung tissue lysate (40 µg/Lane) Lane 9: Human lung tissue lysate (40 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721660) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.