PEDF Recombinant Rabbit Monoclonal Antibody [JE41-62]
cat.: HA721661
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE41-62 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PEDF aa 1-50 / 418. |
| Positive control: | Mouse liver tissue lysate, rat liver tissue lysate. |
| Subcellular location: | Secreted, Melanosome. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P36955 Human | P97298 Mouse Entrez Gene: 287526 Rat |
| Alternative names: | Cell proliferation-inducing gene 35 protein EPC 1 EPC-1 EPC1 OI12 OI6 PEDF PEDF_HUMAN PIG 35 PIG35 Pigment epithelium derived factor Pigment epithelium-derived factor Proliferation inducing protein 35 Serine (or cysteine) proteinase inhibitor serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 Serpin F1 Serpin family F member 1 Serpin peptidase inhibitor Serpin peptidase inhibitor clade F member 1 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 SERPINF 1 Serpinf1 |
Images
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Fig1:
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA721661) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721661) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.