Laminin gamma 1 Recombinant Rabbit Monoclonal Antibody [JE40-75]
cat.: HA721662
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE40-75 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 178 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Laminin gamma 1 aa 21-70 / 1,609. |
| Positive control: | HeLa cell lysate, A431 cell lysate, OVCAR-3 cell lysate, A549 cell lysate. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix, basement membrane. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P11047 Human |
| Alternative names: | LAMB2 lamc1 LAMC1_HUMAN Laminin B2 chain Laminin subunit gamma-1 Laminin, gamma 1 (formerly LAMB2) Laminin-1 subunit gamma Laminin-10 subunit gamma Laminin-11 subunit gamma Laminin-2 subunit gamma Laminin-3 subunit gamma Laminin-4 subunit gamma Laminin-6 subunit gamma Laminin-7 subunit gamma Laminin-8 subunit gamma Laminin-9 subunit gamma MGC87297 S-LAM gamma S-laminin subunit gamma |
Images
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Fig1:
Western blot analysis of Laminin gamma 1 on different lysates with Rabbit anti-Laminin gamma 1 antibody (HA721662) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: OVCAR-3 cell lysate Lane 4: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 178 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721662) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Laminin gamma 1 on different lysates with Rabbit anti-Laminin gamma 1 antibody (HA721662) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Laminin gamma 1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 178 kDa Observed band size: 250 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721662) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.