TCF-4 Recombinant Rabbit Monoclonal Antibody [JE41-73]
cat.: HA721664
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE41-73 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TCF-4 aa 321-570 / 667. |
| Positive control: | A549 cell lysate, NIH/3T3 cell lysate, HepG2 cell lysate, Caco-2 cell lysate, SW620 cell lysate, HeLa cell lysate, 293T cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P15884 Human | Q60722 Mouse |
| Alternative names: | bHLHb19 Class B basic helix-loop-helix protein 19 E2 2 FECD3 Immunoglobulin transcription factor 2 ITF 2 ITF-2 ITF2 ITF2_HUMAN MGC149723 MGC149724 PTHS SEF 2 SEF-2 SEF2 1 SEF2 1A SEF2 1B SEF2 1D SEF2 SL3 3 enhancer factor 2 SL3-3 enhancer factor 2 TCF 4 TCF-4 TCF4 Transcription factor 4 Transcription factor 4, isoform C Transcription factor 4, isoform D Transcription factor 4, isoform E Transcription factor 4, isoform L Transcription factor 4, isoform M Transcription factor 4, isoform R |
Images
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Fig1:
Western blot analysis of TCF-4 on different lysates with Rabbit anti-TCF-4 antibody (HA721664) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: HepG2 cell lysate Lane 4: Caco-2 cell lysate Lane 5: SW620 cell lysate Lane 6: HeLa cell lysate Lane 7: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 71 kDa Observed band size: 55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721664) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.