OLFM4 Recombinant Rabbit Monoclonal Antibody [PSH01-36]
cat.: HA721670
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH01-36 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human OLFM4 aa 1-510 / 510. |
| Positive control: | Human liver tissue. |
| Subcellular location: | Secreted, extracellular space, Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:500 |
| Uniprot #: | SwissProt: Q6UX06 Human |
| Alternative names: | Antiapoptotic protein GW112 bA209J19.1 G-CSF-stimulated clone 1 protein GC1 GW112 hGC 1 hGC-1 hOLfD KIAA4294 olfactomedin 4 Olfactomedin-4 OlfD OLFM4 OLFM4_HUMAN OLM4 UNQ362 |
Images
|
Fig1:
Western blot analysis of OLFM4 on different lysates with Rabbit anti-OLFM4 antibody (HA721670) at 1/1,000 dilution. Lane 1: OLFM4 recombinant protein Lane 2: OLFM4 recombinant protein with immunising peptide Lysates/proteins at 50 ng/Lane. Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721670) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-OLFM4 antibody (HA721670) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721670) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.