Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) Recombinant Rabbit Monoclonal Antibody [PSH01-39]
cat.: HA721673
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: PSH01-39
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 84 kDa/55 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human PIK3R1 aa 451-500 / 724.
Positive control: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate.
Subcellular location: Cell-cell junction, cis-Golgi network, cytoplasm, cytosol, membrane, nucleus.
Recommended Dilutions:
  WB
  IF-Cell

1:1,000
1:100
Uniprot #: SwissProt: P27986 Human | P26450 Mouse
Alternative names: GRB1 p85 alpha p85 P85A_HUMAN Phosphatidylinositol 3 kinase associated p 85 alpha Phosphatidylinositol 3 kinase regulatory 1 Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha Phosphatidylinositol 3-kinase regulatory subunit alpha Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) PI3 kinase p85 PI3 kinase p85 subunit alpha PI3-kinase regulatory subunit alpha PI3-kinase subunit p85-alpha PI3K PI3K p85 PI3K regulatory subunit alpha Pik3r1 PtdIns 3 kinase p85 alpha PtdIns-3-kinase regulatory subunit alpha PtdIns-3-kinase regulatory subunit p85-alpha
Images
HA721673_1.jpg Fig1: Western blot analysis of Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) on different lysates with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody (HA721673) at 1/1,000 dilution.

Lane 1: NIH/3T3 whole cell lysate
Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 84 kDa
Observed band size: 55/85 kDa

Exposure time: 3 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721673) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
HA721673_2.jpg Fig2: Western blot analysis of Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) on different lysates with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody (HA721673) at 1/1,000 dilution.

Lane 1: 293T overexpress Src whole cell lysate
Lane 2: 293T overexpress Src treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate
Lane 3: 293T overexpress Src treated with λpp for 1 hour whole cell lysate
Lane 4: 293T overexpress Src treated with 1mM sodium orthovanadate for 30 minutes, then treated with λpp for 1 hour whole cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 84 kDa
Observed band size: 55 kDa

Exposure time: 28 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721673) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.