Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE58-32]
cat.: HA721676
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE58-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to residues surrounding Ser406 of human eIF4B protein. |
| Positive control: | NIH/3T3 cell lysate, HeLa cell lysate. |
| Subcellular location: | cytosol, eukaryotic translation initiation factor 4F complex. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P23588 Human | Q8BGD9 Mouse | Q5RKG9 Rat |
| Alternative names: | 2310046H11Rik AL024095 C85189 EIF 4B eIF-4B EIF4B Eukaryotic initiation factor 4B Eukaryotic translation initiation factor 4B IF4B_HUMAN PRO1843 |
Images
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Fig1:
Western blot analysis of Phospho-eIF4B (S406) on different lysates with Rabbit anti-Phospho-eIF4B (S406) antibody (HA721676) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: HeLa cell lysate Lane 3: NIH/3T3 treated λpp for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 80 kDa Exposure time: 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721676) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.