HA721678

TMP21 Recombinant Rabbit Monoclonal Antibody [PSH01-40]

cat.: HA721678

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Tissue
Clonality:	Monoclonal
Clone number:	PSH01-40

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 25 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human TMP21 aa 1-200 / 219.
Positive control:	HeLa cell lysate, 293T cell lysate, Raji cell lysate, Jurkat cell lysate, HepG2 cell lysate, Ramos cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, mouse pancreas tissue lysate, mouse liver tissue lysate, rat pancreas tissue lysate, rat liver tissue lysate, human breast tissue, human endometrium tissue, mouse pancreas tissue, rat pancreas tissue.
Subcellular location:	Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane, Golgi apparatus, cis-Golgi network membrane, Golgi apparatus, trans-Golgi network membrane, Cytoplasmic vesicle, secretory vesicle membrane, Cell membrane, Melanosome.
Recommended Dilutions: WB IHC-P IF-Tissue	1:5,000 1:200-1:1,000 1:200
Uniprot #:	SwissProt: P49755 Human \| Q9D1D4 Mouse \| Q63584 Rat
Alternative names:	1110014C03Rik 21 kDa transmembrane trafficking protein 21 kDa transmembrane-trafficking protein MGC102351 p23 p24 family protein delta 1 p24 family protein delta-1 p24(DELTA) p24delta p24delta1 S31I125 S31III125 TMED 10 Tmed10 TMEDA_HUMAN Tmp 21 I Tmp 21 Tmp 21 I Tmp-21-I Transmembrane emp24 domain containing protein 10 Transmembrane emp24 domain-containing protein 10 Transmembrane emp24-like trafficking protein 10 (yeast) Transmembrane protein Tmp21 Transmembrane trafficking protein 21kD

Images

Fig1: Western blot analysis of TMP21 on different lysates with Rabbit anti-TMP21 antibody (HA721678) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: Jurkat cell lysate (20 µg/Lane)
Lane 5: HepG2 cell lysate (20 µg/Lane)
Lane 6: Ramos cell lysate (20 µg/Lane)
Lane 7: U-87 MG cell lysate (20 µg/Lane)
Lane 8: RAW264.7 cell lysate (20 µg/Lane)
Lane 9: Neuro-2a cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Lane 11: Mouse pancreas tissue lysate (40 µg/Lane)
Lane 12: Mouse liver tissue lysate (40 µg/Lane)
Lane 13: Rat pancreas tissue lysate (40 µg/Lane)
Lane 14: Rat liver tissue lysate (40 µg/Lane)

Predicted band size: 25 kDa
Observed band size: 19 kDa

Exposure time: 2 minutes 13 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721678) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-TMP21 antibody (HA721678) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721678) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig3: Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-TMP21 antibody (HA721678) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721678) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-TMP21 antibody (HA721678) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721678) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-TMP21 antibody (HA721678) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721678) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunofluorescence analysis of paraffin-embedded human breast tissue labeling TMP21 with Rabbit anti-TMP21 antibody (HA721678) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721678, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Fig7: Immunofluorescence analysis of paraffin-embedded rat pancreas tissue labeling TMP21 with Rabbit anti-TMP21 antibody (HA721678) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721678, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.