PIP5K1C Recombinant Rabbit Monoclonal Antibody [PSH01-43]
cat.: HA721681
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH01-43 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PIP5K1C aa 31-470 / 668. |
| Positive control: | HeLa cell lysate, K-562 cell lysate, 293T cell lysate, A431 cell lysate, SiHa cell lysate, A549 cell lysate, LoVo cell lysate. |
| Subcellular location: | Cell membrane, Endomembrane system, Cytoplasm, Cell junction, focal adhesion, adherens junction, Cell projection, ruffle membrane, phagocytic cup, uropodium; Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O60331 Human |
| Alternative names: | Diphosphoinositide kinase LCCS3 Phosphatidylinositol-4-phosphate 5-kinase type I gamma Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma PI51C PI51C_HUMAN PIP5K GAMMA PIP5K1-gamma Pip5k1c PIP5KIgamma PtdIns(4)P 5 kinase gamma PtdIns(4)P-5-kinase 1 gamma Type I PIP kinase |
Images
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Fig1:
Western blot analysis of PIP5K1C on different lysates with Rabbit anti-PIP5K1C antibody (HA721681) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: 293T cell lysate Lane 4: A431 cell lysate Lane 5: SiHa cell lysate Lane 6: A549 cell lysate Lane 7: LoVo cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 73 kDa Observed band size: 87/90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721681) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of PIP5K1C on different lysates with Rabbit anti-PIP5K1C antibody (HA721681) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si PIP5K1C cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 73 kDa Observed band size: 87 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721681) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.