RACGAP1 Recombinant Rabbit Monoclonal Antibody [JE41-19]
cat.: HA721687
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE41-19 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human RACGAP1 aa 51-100 / 632. |
| Positive control: | Jurkat cell lysate, K-562 cell lysate, 293T cell lysate, HeLa cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm, cytoskeleton, spindle, Cytoplasmic vesicle, secretory vesicle, acrosome, Cleavage furrow, Midbody, Midbody ring, Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9H0H5 Human | Q9WVM1 Mouse Entrez Gene: 315298 Rat |
| Alternative names: | CYK4 GAP Gap1 GTPase activating protein HsCYK-4 ID GAP KIAA1478 Male germ cell RacGap MgcRacGAP Protein CYK4 homolg Protein CYK4 homolog Rac GTPase activating protein 1 Rac GTPase-activating protein 1 RACGAP 1 Racgap1 RGAP1_HUMAN |
Images
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Fig1:
Western blot analysis of RACGAP1 on different lysates with Rabbit anti-RACGAP1 antibody (HA721687) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: 293T cell lysate Lane 4: HeLa cell lysate Lane 5: PC-12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721687) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.