Myogenin Recombinant Rabbit Monoclonal Antibody [JE60-39]
cat.: HA721688
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE60-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Myogenin aa 1-50 / 224. |
| Positive control: | Differentiated C2C12 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P15173 Human | P12979 Mouse | P20428 Rat |
| Alternative names: | bHLHc3 cb553 Class C basic helix-loop-helix protein 3 MYF 4 Myf-4 MYF4 MYOG MYOG_HUMAN Myogenic factor 4 Myogenic factor 4 myogenin Myogenin Myogenin myogenin factor 4 OTTHUMP00000039094 |
Images
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Fig1:
Western blot analysis of Myogenin on different lysates with Rabbit anti-Myogenin antibody (HA721688) at 1/1,000 dilution. Lane 1: Undifferentiated C2C12 whole cell lysate Lane 2: Differentiated C2C12 for 1 day whole cell lysate Lane 3: Differentiated C2C12 for 2 days whole cell lysate Lane 4: Differentiated C2C12 for 3 days whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 35 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.