CD200 Recombinant Rabbit Monoclonal Antibody [PSH01-49]
cat.: HA721692
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: PSH01-49
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 31 kDa
Isotype: IgG
Immunogen: Recombinant protein within human CD200 aa 1-259 / 278.
Positive control: SK-MEL-28 cell lysate, NCI-H226 cell lysate, human brain tissue lysate, human lung tissue lysate, human lymph node tissue, human placenta tissue, SK-MEL-28, NCI-H226.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:1,000
1:2,000
1:500-1:1,000
Uniprot #: SwissProt: P41217 Human
Alternative names: Antigen identified by monoclonal MRC OX 2 CD200 CD200 antigen CD200 molecule MOX1 MOX2 MRC MRC OX 2 antigen My033 OX 2 OX 2 membrane glycoprotein precursor OX-2 membrane glycoprotein OX2G OX2G_HUMAN
Images
HA721692_1.jpg Fig1: Western blot analysis of CD200 on different lysates with Rabbit anti-CD200 antibody (HA721692) at 1/1,000 dilution.

Lane 1: SK-MEL-28 cell lysate
Lane 2: NCI-H226 cell lysate
Lane 3: Human brain tissue lysate
Lane 4: Human lung tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 31 kDa
Observed band size: 40-50kDa

Exposure time: 2 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721692) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
HA721692_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-CD200 antibody (HA721692) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721692) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721692_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD200 antibody (HA721692) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721692) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721692_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue (negative control) with Rabbit anti-CD200 antibody (HA721692) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721692) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721692_5.jpg Fig5: Flow cytometric analysis of SK-MEL-28 cells labeling CD200.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721692, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721692_6.jpg Fig6: Flow cytometric analysis of NCI-H226 cells labeling CD200.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721692, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.