Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | PSH01-50 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 65 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human TGF beta Receptor II aa 23-166 (Extracellular). |
Positive control: | HepG2 cell lysate, A549 cell lysate, HT-29 cell lysate. |
Subcellular location: | Cell membrane, Membrane raft; Secreted. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: P37173 Human |
Alternative names: | AAT3 FAA3 LDS1B LDS2 LDS2B MFS2 RIIC TAAD2 TbetaR II TbetaR-II TGF beta receptor type 2 TGF beta receptor type II TGF beta receptor type IIB TGF beta type II receptor TGF-beta receptor type II TGF-beta receptor type-2 TGF-beta type II receptor TGF-beta-R2 TGFB R2 TGFbeta - RII TGFbeta RII Tgfbr2 TGFR-2 TGFR2_HUMAN Transforming growth factor beta receptor II Transforming growth factor beta receptor type II Transforming growth factor beta receptor type IIC Transforming growth factor, beta receptor II (70/80kDa) transforming growth factor, beta receptor II alpha transforming growth factor, beta receptor II beta transforming growth factor, beta receptor II delta transforming growth factor, beta receptor II epsilon transforming growth factor, beta receptor II gamma Transforming growth factor-beta receptor type II |
Fig1:
Western blot analysis of TGF beta Receptor II on different lysates with Rabbit anti-TGF beta Receptor II antibody (HA721693) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate Lane 3: HT-29 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 65 kDa Observed band size: 65-80 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721693) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |