MCAK Recombinant Rabbit Monoclonal Antibody [PSH01-51]
cat.: HA721694
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH01-51 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 81.3 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MCAK aa 200-650. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, K-562 cell lysate, Jurkat cell lysate, 293T cell lysate, human testis tissue, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q99661 Human |
| Alternative names: | 4930402F02Rik CT139 ESTM5 KIF 2C kif2c KIF2C_HUMAN Kinesein Family Member 2C Kinesin family member 2C kinesin like 6 (mitotic centromere associated kinesin) Kinesin like 6 Kinesin like protein 6 Kinesin like protein KIF2C Kinesin-like protein 6 Kinesin-like protein KIF2C KNS L6 KNSL 6 KNSL6 MCAK MGC11883 Mitotic centromere associated kinesin Mitotic centromere-associated kinesin OTTHUMP00000010066 X83316 |
Images
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Fig1:
Western blot analysis of MCAK on different lysates with Rabbit anti-MCAK antibody (HA721694) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721694) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of MCAK on different lysates with Rabbit anti-MCAK antibody (HA721694) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-MCAK KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721694) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-MCAK antibody (HA721694) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721694) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-MCAK antibody (HA721694) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721694) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-MCAK antibody (HA721694) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721694) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.