DOT1L Recombinant Rabbit Monoclonal Antibody [PSH01-57]
cat.: HA721700
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH01-57 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 165 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DOT1/KMT4 aa 1- 500. |
| Positive control: | K-562 cell lysate, THP-1 cell lysate, HeLa cell lysate, 293T cell lysate, human testis tissue, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q8TEK3 Human |
| Alternative names: | Disrupter of telomere silencing protein 1 DOT 1 DOT1 DOT1 like DOT1 like histone H3 methyltransferase (S. cerevisiae) DOT1 like histone H3 methyltransferase DOT1 like histone H3K79 methyltransferase DOT1 like protein DOT1, S. cerevisiae, homolog of DOT1-like protein DOT1L DOT1L_HUMAN EC 2.1.1.43 H3 K79 HMTase H3 lysine-79 specific H3-K79-HMTase Histone H3 K79 methyltransferase Histone H3 methyltransferase Histone H3 methyltransferase DOT1-like Histone H3-K79 methyltransferase Histone lysine N methyltransferase H3 lysine 79 specific Histone methyltransferase DOT1L Histone-lysine N-methyltransferase KIAA1814 KMT4 Lysine N methyltransferase 4 Lysine N-methyltransferase 4 |
Images
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Fig1:
Western blot analysis of DOT1L on different lysates with Rabbit anti-DOT1L antibody (HA721700) at 1/1,000 dilution. Lane 1: K-562 cell lysate (40 µg/Lane) Lane 2: THP-1 cell lysate (40 µg/Lane) Lane 3: HeLa cell lysate (40 µg/Lane) Lane 4: 293T cell lysate (40 µg/Lane) Predicted band size: 165 kDa Observed band size: 180 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DOT1L antibody (HA721700) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721700) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-DOT1L antibody (HA721700) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721700) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-DOT1L antibody (HA721700) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721700) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.