PSMB10 Recombinant Rabbit Monoclonal Antibody [PSH01-58]
cat.: HA721701
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH01-58 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PSMB10 aa 1-273. |
| Positive control: | HeLa treated with 100ng/mL IFN-γ for 48 hours cell lysate, HeLa cell lysate, Daudi cell lysate, Raji cell lysate, THP-1 cell lysate, Ramos cell lysate, U-937 cell lysate, HL-60 cell lysate, Jurkat cell lysate, SiHa cell lysate, mouse spleen tissue lysate, mouse thymus tissue lysate, human lung tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P40306 Human | O35955 Mouse |
| Alternative names: | beta2i FLJ00366 LMP10 Low molecular mass protein 10 Macropain subunit MECl 1 Macropain subunit MECl-1 MECL1 MGC1665 Multicatalytic endopeptidase complex subunit MECl 1 Multicatalytic endopeptidase complex subunit MECl-1 OTTHUMP00000174858 Proteasome (prosome macropain) subunit beta type 10 Proteasome beta 10 subunit Proteasome catalytic subunit 2i Proteasome MECl 1 Proteasome MECl-1 Proteasome subunit beta 2i Proteasome subunit beta 7i Proteasome subunit beta type 10 Proteasome subunit beta type-10 Proteasome subunit beta-2i Proteasome subunit MECL1 PSB10_HUMAN PSMB 10 Psmb10 |
Images
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Fig1:
Western blot analysis of PSMB10 on different lysates with Rabbit anti-PSMB10 antibody (HA721701) at 1/1,000 dilution. Lane 1: HeLa treated with 100ng/mL IFN-γ for 48 hours cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Daudi cell lysate (20 µg/Lane) Lane 4: Raji cell lysate (20 µg/Lane) Lane 5: THP-1 cell lysate (20 µg/Lane) Lane 6: Ramos cell lysate (20 µg/Lane) Lane 7: U-937 cell lysate (20 µg/Lane) Lane 8: HL-60 cell lysate (20 µg/Lane) Lane 9: Jurkat cell lysate (20 µg/Lane) Lane 10: SiHa cell lysate (20 µg/Lane) Lane 11: Mouse spleen tissue lysate (40 µg/Lane) Lane 12: Mouse thymus tissue lysate (40 µg/Lane) Predicted band size: 29 kDa Observed band size: 25/29 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721701) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PSMB10 antibody (HA721701) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721701) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.