KPNB1 Recombinant Rabbit Monoclonal Antibody [PSH01-59]
cat.: HA721702
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH01-59 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97.2 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human KPNB1 aa 1-550. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, A431 cell lysate, A549 cell lysate, Jurkat cell lysate, K-562 cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, mouse spleen tissue lysate, mouse testis tissue lysate, rat spleen tissue lysate, rat testis tissue lysate. |
| Subcellular location: | Cytoplasm. Nucleus envelope. |
| Recommended Dilutions:
WB IP |
1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q14974 Human | P70168 Mouse | P52296 Rat |
| Alternative names: | IMB 1 IMB1 IMB1_HUMAN Impnb Importin 90 Importin beta 1 Importin beta 1 subunit Importin subunit beta-1 Importin-90 IPOB Karyopherin beta 1 Karyopherin beta 1 subunit Karyopherin subunit beta-1 KPNB 1 Kpnb1 MGC2155 MGC2156 MGC2157 NTF 97 NTF97 NTF97/Importin beta Nuclear factor P97 Pore targeting complex 97 kDa subunit PTAC97 |
Images
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Fig1:
Western blot analysis of KPNB1 on different lysates with Rabbit anti-KPNB1 antibody (HA721702) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (20 µg/Lane) Lane 7: Caco-2 cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: PC-12 cell lysate (20 µg/Lane) Lane 10: C6 cell lysate (20 µg/Lane) Lane 11: Mouse spleen tissue lysate (40 µg/Lane) Lane 12: Mouse testis tissue lysate (40 µg/Lane) Lane 13: Rat spleen tissue lysate (40 µg/Lane) Lane 14: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721702) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
KPNB1 was immunoprecipitated in 0.2mg HeLa cell lysate with HA721702 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA721702 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of HA721702 in HeLa cell lysate Lane 3: HA721702 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.