PICALM Recombinant Rabbit Monoclonal Antibody [PSH01-63]
cat.: HA721706
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH01-63 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PICALM aa 1-450. |
| Positive control: | HepG2 cell lysate, PC-3M cell lysate, A431 cell lysate, K-562 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human breast tissue, human placenta tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cell membrane, Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane, Nucleus |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:50 |
| Uniprot #: | SwissProt: Q13492 Human | Q7M6Y3 Mouse | O55012 Rat |
| Alternative names: | CALM Clathrin assembly lymphoid myeloid leukemia Clathrin assembly lymphoid myeloid leukemia protein CLTH LAP Phosphatidylinositol-binding clathrin assembly protein PICAL_HUMAN PICALM |
Images
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Fig1:
Western blot analysis of PICALM on different lysates with Rabbit anti-PICALM antibody (HA721706) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: PC-3M cell lysate Lane 3: A431 cell lysate Lane 4: K-562 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 71 kDa Observed band size: 65/71 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721706) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-PICALM antibody (HA721706) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721706) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-PICALM antibody (HA721706) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721706) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PICALM antibody (HA721706) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721706) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PICALM antibody (HA721706) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721706) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.