PICALM Recombinant Rabbit Monoclonal Antibody [PSH01-64]
cat.: HA721707
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH01-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PICALM aa 1-450. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, PC-3M cell lysate, HCT 116 cell lysate, 293T cell lysate, Neuro-2a cell lysate, PC-12 cell lysate. |
| Subcellular location: | Cell membrane, Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane, Nucleus |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q13492 Human | Q7M6Y3 Mouse | O55012 Rat |
| Alternative names: | CALM Clathrin assembly lymphoid myeloid leukemia Clathrin assembly lymphoid myeloid leukemia protein CLTH LAP Phosphatidylinositol-binding clathrin assembly protein PICAL_HUMAN PICALM |
Images
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Fig1:
Western blot analysis of PICALM on different lysates with Rabbit anti-PICALM antibody (HA721707) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: PC-3M cell lysate Lane 4: HCT 116 cell lysate Lane 5: 293T cell lysate Lane 6: Neuro-2a cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 71 kDa Observed band size: 65/71 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721707) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.