IKK beta Recombinant Rabbit Monoclonal Antibody [JE32-81]
cat.: HA721715
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE32-81 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IKK beta aa 1-50 / 756. |
| Positive control: | K-562 cell lysate, HL-60 cell lysate, THP-1 cell lysate, Jurkat cell lysate, HeLa cell lysate, THP-1. |
| Subcellular location: | Cytoplasm, Nucleus, Membrane raft. |
| Recommended Dilutions:
WB FC |
1:1,000 1:50 |
| Uniprot #: | SwissProt: O14920 Human |
| Alternative names: | I kappa B kinase 2 I kappa B kinase beta I-kappa-B kinase 2 I-kappa-B-kinase beta IkBKB IKK beta IKK-B IKK-beta IKK2 IKKB IKKB_HUMAN IMD15 Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta Inhibitor of nuclear factor kappa-B kinase subunit beta NFKBIKB Nuclear factor NF-kappa-B inhibitor kinase beta |
Images
|
Fig1:
Western blot analysis of IKK beta on different lysates with Rabbit anti-IKK beta antibody (HA721715) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lane 4: Jurkat cell lysate Lane 5: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 87 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721715) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of IKK beta on different lysates with Rabbit anti-IKK beta antibody (HA721715) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IKK beta KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 87 kDa Observed band size: 87 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721715) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Flow cytometric analysis of THP-1 cells labeling IKK beta. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721715, 1/50) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.