Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE36-10 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 66 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human PTH1R aa 39-188 / 593. |
Positive control: | HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, THP-1 cell lysate. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q03431 Human |
Alternative names: | MGC138426 MGC138452 Parathyroid hormone 1 receptor Parathyroid hormone/parathyroid hormone related peptide receptor Parathyroid hormone/parathyroid hormone related protein receptor Parathyroid hormone/parathyroid hormone-related peptide receptor PTH receptor PTH/PTHr receptor PTH/PTHrP receptor PTH/PTHrP type I receptor PTH1 receptor PTH1R PTH1R_HUMAN PTHR 1 PTHR PTHR1 |
Fig1:
Western blot analysis of PTH1R on different lysates with Rabbit anti-PTH1R antibody (HA721716) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF7 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721716) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |