| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE36-10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PTH1R aa 39-188 / 593. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, THP-1 cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q03431 Human |
| Alternative names: | MGC138426 MGC138452 Parathyroid hormone 1 receptor Parathyroid hormone/parathyroid hormone related peptide receptor Parathyroid hormone/parathyroid hormone related protein receptor Parathyroid hormone/parathyroid hormone-related peptide receptor PTH receptor PTH/PTHr receptor PTH/PTHrP receptor PTH/PTHrP type I receptor PTH1 receptor PTH1R PTH1R_HUMAN PTHR 1 PTHR PTHR1 |
|
Fig1:
Western blot analysis of PTH1R on different lysates with Rabbit anti-PTH1R antibody (HA721716) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF7 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721716) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |