PTH1R Recombinant Rabbit Monoclonal Antibody [JE36-10]
cat.: HA721716
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE36-10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 66 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human PTH1R aa 39-188 / 593.
Positive control: HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, THP-1 cell lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q03431 Human
Alternative names: MGC138426 MGC138452 Parathyroid hormone 1 receptor Parathyroid hormone/parathyroid hormone related peptide receptor Parathyroid hormone/parathyroid hormone related protein receptor Parathyroid hormone/parathyroid hormone-related peptide receptor PTH receptor PTH/PTHr receptor PTH/PTHrP receptor PTH/PTHrP type I receptor PTH1 receptor PTH1R PTH1R_HUMAN PTHR 1 PTHR PTHR1
Images
HA721716_1.jpg Fig1: Western blot analysis of PTH1R on different lysates with Rabbit anti-PTH1R antibody (HA721716) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: THP-1 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 66 kDa
Observed band size: 66 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721716) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.