AP-2 complex subunit alpha-1 Recombinant Rabbit Monoclonal Antibody [JE40-38]
cat.: HA721717
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE40-38 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 108 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human AP-2 complex subunit alpha-1 aa 1-100 / 977. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, human brain tissue. |
| Subcellular location: | Cell membrane, Membrane, coated pit. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: O95782 Human | P17426 Mouse Entrez Gene: 308578 Rat |
| Alternative names: | 100 kDa coated vesicle protein A Adapter-related protein complex 2 alpha-1 subunit adapter-related protein complex 2 subunit alpha-1 Adaptin alpha A Adaptin alpha Adaptor protein complex AP 2 alpha 1 subunit adaptor protein complex AP 2 subunit alpha 1 Adaptor protein complex AP-2 subunit alpha-1 Adaptor protein complex AP2 alpha 1 subunit adaptor protein complex AP2 subunit alpha 1 Adaptor related protein complex 2 alpha 1 subunit Adaptor related protein complex AP 2 alpha 1 subunit Adaptor related protein complex AP2 alpha 1 subunit ADTAA Alpha A adaptin Alpha adaptin A Alpha-adaptin A alpha1 adaptin Alpha1-adaptin AP 2 alpha AP 2 complex subunit alpha 1 AP-2 complex subunit alpha-1 AP2 complex subunit alpha 1 AP2-ALPHA Ap2a1 AP2A1_HUMAN CLAPA 1 CLAPA1 Clathrin adaptor complex AP2. alpha subunit Clathrin assembly protein complex 2 alpha A large chain Clathrin assembly protein complex 2 alpha-A large chain Clathrin associat...... |
Images
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Fig1:
Western blot analysis of AP-2 complex subunit alpha-1 on different lysates with Rabbit anti-AP-2 complex subunit alpha-1 antibody (HA721717) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 108 kDa Observed band size: 108 kDa Exposure time: 10 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721717) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-AP-2 complex subunit alpha-1 antibody (HA721717) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721717) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.