COL11A1 Recombinant Rabbit Monoclonal Antibody [JE40-84]
cat.: HA721718
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE40-84 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 181 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human COL11A1 aa 501-600 / 1,806. |
| Positive control: | K-562 cell lysate, Jurkat cell lysate, PANC-1 cell lysate, Saos-2 cell lysate. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P12107 Human |
| Alternative names: | COBA1_HUMAN COL11A1 COLL6 Collagen alpha 1 Collagen alpha-1(XI) chain collagen XI alpha 1 collagen XI, alpha 1 polypeptide collagen, type XI, alpha 1 STL2 STL3 XI chain precursor |
Images
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Fig1:
Western blot analysis of COL11A1 on different lysates with Rabbit anti-COL11A1 antibody (HA721718) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: PANC-1 cell lysate Lane 4: Saos-2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 181 kDa Observed band size: 150 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721718) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.