Melanoma gp100 Recombinant Rabbit Monoclonal Antibody [JE42-47]
cat.: HA721719
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE42-47 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Melanoma gp100 aa 617-661 / 661. |
| Positive control: | SK-MEL-28 cell lysates, human melanoma tissue, mouse testis tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, Golgi apparatus, cis-Golgi network membrane, Endosome, multivesicular body, Melanosome, Extracellular vesicle, Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P40967 Human | Q60696 Mouse |
| Alternative names: | 95 kDa melanocyte specific secreted glycoprotein 95 kDa melanocyte-specific secreted glycoprotein D12S53E gp100 M-beta ME20 ME20 M/ME20 S ME20-M ME20-S ME20M ME20M/ME20S ME20S Melanocyte lineage specific antigen GP100 Melanocyte protein mel 17 Melanocyte protein Pmel 17 Melanocyte protein Pmel 17 precursor Melanocytes lineage-specific antigen GP100 Melanoma associated ME20 antigen Melanoma gp100 Melanoma-associated ME20 antigen Melanosomal matrix protein 17 Melanosomal matrix protein17 P1 p100 p26 PMEL 17 PMEL PMEL_HUMAN PMEL17 Premelanosome protein Secreted melanoma-associated ME20 antigen SI SIL SILV Silver (mouse homolog) like Silver homolog Silver locus protein homolog Silver, mouse, homolog of |
Images
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Fig1:
Western blot analysis of Melanoma gp100 on SK-MEL-28 cell lysates with Rabbit anti-Melanoma gp100 antibody (HA721719) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 70 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721719) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human melanoma tissue with Rabbit anti-Melanoma gp100 antibody (HA721719) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721719) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Melanoma gp100 antibody (HA721719) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721719) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.