ErbB4 Recombinant Rabbit Monoclonal Antibody [JE59-43]
cat.: HA721720
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE59-43
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 147 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ErbB4 aa 1,231-1,280 / 1,308.
Positive control: Mouse brain tissue lysate, rat brain tissue lysate.
Subcellular location: Cell membrane; Nucleus, Mitochondrion.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q15303 Human | Q61527 Mouse | Q62956 Rat
Alternative names: 4ICD ALS19 Avian erythroblastic leukemia viral oncogene homolog 4 Avian erythroblastic leukemia viral v erb b2 oncogene homolog 4 E4ICD EC 2.7.10.1 ErbB 4 Erbb4 ERBB4 intracellular domain ERBB4_HUMAN HER 4 HER4 human epidermal growth factor receptor 4 Mer4 MGC138404 Oncogene ERBB4 p180erbB4 Proto-oncogene-like protein c-ErbB-4 Receptor protein tyrosine kinase erbB 4 precursor Receptor tyrosine protein kinase erbB 4 s80HER4 Tyrosine kinase type cell surface receptor HER4 Tyrosine kinase-type cell surface receptor HER4 v erb a avian erythroblastic leukemia viral oncogene homolog like 4 v erb a erythroblastic leukemia viral oncogene homolog 4 v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) V-ERB-B2 avian erythroblastic leukemia viral oncogene homolog 4 Verba avian erythroblastic leukemia viral oncogene homolog like 4 Verba erythroblastic leukemia viral oncogene homolog 4 VERBB2
Images
HA721720_1.jpg Fig1: Western blot analysis of ErbB4 on different lysates with Rabbit anti-ErbB4 antibody (HA721720) at 1/1,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 147 kDa
Observed band size: 180 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721720) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.