Human IL-18 Recombinant Rabbit Monoclonal Antibody [PSH01-71]
cat.: HA721722
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH01-71 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22.3 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-18 aa 37-193. |
| Positive control: | Recombinant human IL-18 protein (HA210779). |
| Subcellular location: | Cytoplasm, Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH01-70] to Human IL-18 (Detector) (HA721723). The reference range value is 15.6-3800 pg/ml. |
| Uniprot #: | SwissProt: Q14116 Human |
| Alternative names: | Iboctadekin IFN gamma inducing factor IFN-gamma-inducing factor IGIF IL 1 gamma IL 18 IL 1g IL-1 gamma IL-18 IL1 gamma IL18 IL18 protein IL18_HUMAN IL1F4 IL1g IL1gamma ILIF4 Interferon gamma inducing factor Interferon gamma-inducing factor Interleukin 1 gamma Interleukin 18 (interferon-gamma-inducing factor) Interleukin 18 Interleukin-1 gamma Interleukin-18 Interleukin18 MGC12320 |
Images
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Fig1:
Sandwich ELISA analysis of human IL-18 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721722) diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human IL-18 protein starting from 3800 pg/ml to 0 pg/ml and detect antibody [PSH01-70]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.