CLP Recombinant Rabbit Monoclonal Antibody [PSH01-72]
cat.: HA721724
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH01-72 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CLP aa 1-170 / 170. |
| Positive control: | Human spleen tissue, mouse spleen tissue, rat spleen tissue. |
| Subcellular location: | Secreted, Vesicle. |
| Recommended Dilutions:
IHC IF-Tissue |
1:1,000-1:2,000 1:50 |
| Uniprot #: | SwissProt: P49913 Human | P51437 Mouse | G3V8S9 Rat |
| Alternative names: | 18 kDa cationic antimicrobial protein Antibacterial peptide LL-37 Antibacterial protein FALL-39 CAMP CAMP_HUMAN CAP 18 CAP-18 CAP18 Cathelicidin antimicrobial peptide Cathelin-like protein Cathelin-related antimicrobial peptide CATHL3 Cationic antimicrobial protein, 18-KD CLP Cnlp Cramp CRAMP, mouse, homolog of FALL 39 FALL-39 peptide antibiotic FALL39 hCAP 18 hCAP-18 hCAP18 HSD26 LL37 MCLP Peptide antibiotic, PR-39, porcine, homolog of |
Images
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CLP antibody (HA721724) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721724) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CLP antibody (HA721724) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721724) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CLP antibody (HA721724) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721724) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human brain tissue (negative) with Rabbit anti-CLP antibody (HA721724) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721724) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling CLP with Rabbit anti-CLP antibody (HA721724) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721724, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.