SNAP29 Recombinant Rabbit Monoclonal Antibody [PSH01-76]
cat.: HA721728
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH01-76 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SNAP29 aa 1-258 / 258. |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, U-87 MG cell lysate. |
| Subcellular location: | Cytoplasm, Golgi apparatus membrane, Cytoplasmic vesicle, autophagosome membrane, Cell projection, cilium membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O95721 Human |
| Alternative names: | CEDNIK FLJ21051 SNAP 29 SNAP-29 SNAP29 SNP29_HUMAN Soluble 29 kDa NSF attachment protein Synaptosomal associated protein 29 Synaptosomal associated protein 29kDa Synaptosomal-associated protein 29 Vesicle membrane fusion protein SNAP 29 Vesicle membrane fusion protein SNAP29 Vesicle-membrane fusion protein SNAP-29 |
Images
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Fig1:
Western blot analysis of SNAP29 on different lysates with Rabbit anti-SNAP29 antibody (HA721728) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: A549 cell lysate Lane 5: U-87 MG cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721728) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.