KDM3A / JHDM2A Recombinant Rabbit Monoclonal Antibody [PSH01-77]
cat.: HA721729
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC |
| Clonality: | Monoclonal |
| Clone number: | PSH01-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 147 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human KDM3A aa 1-400 / 1,321. |
| Positive control: | HCT116 cell lysate, Hela cell lysate, MCF7 cell lysate, Ramos cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, human testis tissue, mouse testis tissue. |
| Subcellular location: | Cytoplasm, Nucleus |
| Recommended Dilutions:
WB IHC |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q9Y4C1 Human | Q6PCM1 Mouse |
| Alternative names: | DKFZp686A24246 DKFZp686P07111 JHDM2A JHMD2A JmjC domain-containing histone demethylation protein 2A JMJD1 JMJD1A Jumonji C domain containing histone demethylase 2A Jumonji domain containing 1 Jumonji domain containing 1A Jumonji domain containing protein 1A Jumonji domain-containing protein 1A Kdm3a KDM3A_HUMAN KIAA0742 Lysine (K) specific demethylase 3A Lysine-specific demethylase 3A Testis specific protein A TSGA |
Images
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Fig1:
Western blot analysis of KDM3A / JHDM2A on different lysates with Rabbit anti-KDM3A / JHDM2A antibody (HA721729) at 1/1,000 dilution. Lane 1: HCT116 cell lysate Lane 2: Hela cell lysate Lane 3: MCF7 cell lysate Lane 4: Ramos cell lysate Lane 5: Jurkat cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 147 kDa Observed band size: 150 kDa Exposure time: 2 minutes 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721729) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KDM3A / JHDM2A antibody (HA721729) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721729) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-KDM3A / JHDM2A antibody (HA721729) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721729) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.