Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IHC |
Clonality: | Monoclonal |
Clone number: | PSH01-77 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 147 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human KDM3A aa 1-400 / 1,321. |
Positive control: | HCT116 cell lysate, Hela cell lysate, MCF7 cell lysate, Ramos cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, human testis tissue, mouse testis tissue. |
Subcellular location: | Cytoplasm, Nucleus |
Recommended Dilutions:
WB IHC |
1:1,000 1:200-1:1,000 |
Uniprot #: | SwissProt: Q9Y4C1 Human | Q6PCM1 Mouse |
Alternative names: | DKFZp686A24246 DKFZp686P07111 JHDM2A JHMD2A JmjC domain-containing histone demethylation protein 2A JMJD1 JMJD1A Jumonji C domain containing histone demethylase 2A Jumonji domain containing 1 Jumonji domain containing 1A Jumonji domain containing protein 1A Jumonji domain-containing protein 1A Kdm3a KDM3A_HUMAN KIAA0742 Lysine (K) specific demethylase 3A Lysine-specific demethylase 3A Testis specific protein A TSGA |
Fig1:
Western blot analysis of KDM3A / JHDM2A on different lysates with Rabbit anti-KDM3A / JHDM2A antibody (HA721729) at 1/1,000 dilution. Lane 1: HCT116 cell lysate Lane 2: Hela cell lysate Lane 3: MCF7 cell lysate Lane 4: Ramos cell lysate Lane 5: Jurkat cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 147 kDa Observed band size: 150 kDa Exposure time: 2 minutes 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721729) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KDM3A / JHDM2A antibody (HA721729) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721729) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-KDM3A / JHDM2A antibody (HA721729) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721729) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |