Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | PSH01-78 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 67 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within mouse MBD1 aa 1-200 / 636. |
Positive control: | HeLa cell lysate, 293T cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, human kidney tissue lysate, mouse kidney tissue lysate, mouse brain tissue lysate, mouse testis tissue lysate, mouse lung tissue lysate, rat brain tissue lysate, rat testis tissue lysate. |
Subcellular location: | Nucleus, Nucleus matrix, Nucleus speckle, Chromosome |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q9UIS9 Human | Q9Z2E2 Mouse | Q66HB8 Rat |
Alternative names: | CXXC 3 CXXC-type zinc finger protein 3 CXXC3 MBD 1 Mbd1 MBD1_HUMAN MECP1 COMPLEX Methyl CpG binding domain protein 1 Methyl CpG binding domain protein 1 isoform PCM1 Methyl CpG binding protein MBD1 Methyl CpG binding protein splice variant 1 Methyl CpG binding protein splice variant 2 Methyl CpG binding protein splice variant 3 Methyl CpG binding protein splice variant 4 Methyl-CpG-binding domain protein 1 Methyl-CpG-binding protein MBD1 PCM 1 PCM1 Protein containing methyl-CpG-binding domain 1 Regulator of fibroblast growth factor 2 (FGF 2) transcription RFT The regulator of fibroblast growth factor 2(FGF 2) transcription |
Fig1:
Western blot analysis of MBD1 on different lysates with Rabbit anti-MBD1 antibody (HA721730) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: Human kidney tissue lysate Lane 6: Mouse kidney tissue lysate Lane 7: Mouse brain tissue lysate Lane 8: Mouse testis tissue lysate Lane 9: Mouse lung tissue lysate Lane 10: Rat brain tissue lysate Lane 11: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721730) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |