MBD1 Recombinant Rabbit Monoclonal Antibody [PSH01-79]
cat.: HA721731
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH01-79 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse MBD1 aa 1-200 / 636. |
| Positive control: | NIH/3T3 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, mouse kidney tissue lysate, mouse brain tissue lysate, mouse lung tissue lysate, mouse spleen tissue lysate, rat brain tissue lysate, NIH/3T3. |
| Subcellular location: | Nucleus, Nucleus matrix, Nucleus speckle, Chromosome |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q9Z2E2 Mouse | Q66HB8 Rat |
| Alternative names: | CXXC 3 CXXC-type zinc finger protein 3 CXXC3 MBD 1 Mbd1 MBD1_HUMAN MECP1 COMPLEX Methyl CpG binding domain protein 1 Methyl CpG binding domain protein 1 isoform PCM1 Methyl CpG binding protein MBD1 Methyl CpG binding protein splice variant 1 Methyl CpG binding protein splice variant 2 Methyl CpG binding protein splice variant 3 Methyl CpG binding protein splice variant 4 Methyl-CpG-binding domain protein 1 Methyl-CpG-binding protein MBD1 PCM 1 PCM1 Protein containing methyl-CpG-binding domain 1 Regulator of fibroblast growth factor 2 (FGF 2) transcription RFT The regulator of fibroblast growth factor 2(FGF 2) transcription |
Images
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Fig1:
Western blot analysis of MBD1 on different lysates with Rabbit anti-MBD1 antibody (HA721731) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lane 3: C2C12 cell lysate Lane 4: Mouse kidney tissue lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse lung tissue lysate Lane 7: Mouse spleen tissue lysate Lane 8: Rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721731) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling MBD1 with Rabbit anti-MBD1 antibody (HA721731) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MBD1 antibody (HA721731) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of NIH/3T3 cells labeling MBD1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721731, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.