SIAH1 Recombinant Rabbit Monoclonal Antibody [PSH01-80]
cat.: HA721732
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH01-80 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SIAH1 aa 1-50 / 282. |
| Positive control: | HCT 116 cell lysate, RAW264.7 cell lysate, mouse liver tissue lysate, mouse brain tissue lysate, rat liver tissue lysate, rat brain tissue lysate, Rat testis testis lysate, A549. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q8IUQ4 Human | P61092 Mouse | Q920M9 Rat |
| Alternative names: | E3 ubiquitin-protein ligase SIAH1 FLJ08065 hSIAH1 HUMSIAH Seven in absentia homolog 1 (Drosophila) Seven in absentia homolog 1 Siah 1 Siah 1a Siah E3 ubiquitin protein ligase 1 Siah-1 Siah-1a Siah1 SIAH1_HUMAN SIAH1A Ubiquitin ligase SIAH1 |
Images
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Fig1:
Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (HA721732) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate（20 µg/Lane） Lane 2: RAW264.7 cell lysate（20 µg/Lane） Lane 3: Mouse liver tissue lysate（40 µg/Lane） Lane 4: Mouse brain tissue lysate（40 µg/Lane） Lane 5: Rat liver tissue lysate（40 µg/Lane） Lane 6: Rat brain tissue lysate（40 µg/Lane） Lane 7: Rat testis testis lysate（40 µg/Lane） Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721732) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (HA721732) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HeLa cell lysate Lane 3: A549 cell lysate Lane 4: HCT116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31/25kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721732) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of A549 cells labeling SIAH1 with Rabbit anti-SIAH1 antibody (HA721732) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIAH1 antibody (HA721732) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.